Optimization of Process Variables to Minimize Uncertainty in Micro-Volume Measurements

Abstract

Calibration of micropipettes plays a significant role in ensuring the reliability and accuracy of volumetric results while conducting testing and research in the areas of biology, analytical chemistry and, pharmaceutical sciences, etc. Understanding the uncertainty elements and their contribution to measurements is the preliminary step in any calibration activity. In the study entitled ’Optimization of process variable to minimize uncertainty in micro-volume measurements’, an attempt is made to analyze the role of uncertainty contributing process variables in volume calibrations based on ISO 8655. The objectives were to identify process variables contributing to the expanded uncertainty and optimization of the variables to minimize the uncertainty. The principles of Design of Experiment (DoE) was the tool applied for the study. The results of the study conclude the role of process variables in the controlled environment for micro-volume calibrations