Optimization of Process Parameters of Extraction of Amentoflavone, Quercetin and Ginkgetin from Taxus chinensis Using Supercritical CO2 Plus Co-Solvent

Xiao Ruan,Liu-Ye Yan,Ben Liu,Qiang Wang,Huan Zhang,Xian-Xian Li

doi:10.3390/molecules191117682

Xiao Ruan, Liu-Ye Yan + Show 4 more

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https://doi.org/10.3390/molecules191117682

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Abstract

The effects of extraction time, temperature, pressure and different concentration of ethanol and their interactions on the yields of amentoflavone, quercetin and ginkgetin extracted from Taxus chinensis by supercritical CO2 were investigated by using a central composite design (CCD). An CCD experimental design with four factors and five levels was used to optimize the extraction parameters. Ultra performance liquid chromatography (UPLC) was used to analyze the content of the tree components in the extracts. Experimental results show that the main effects of factors and their interactions are significant on the yields (p < 0.05). The optimal extraction conditions were established for the three compounds: yield of 4.47 mg/g for amentoflavone at 48 °C, 25 MPa, 2.02 h and 78.5% ethanol, 3.73 mg/g for quercetin at 46 °C, 24 MPa, 2.3 h, 82% ethanol and 3.47 mg/g for ginkgetin at 48 °C, 20 MPa, 2.38 h, 82% ethanol, respectively.

Highlights

The flavonoids are one of the largest known groups of natural products, which are widely distributed in various plants [1,2]
Supercritical CO2 extraction is currently regarded as a natural and green technique for natural product extraction, and an important alternative to conventional separation methods, because it is simpler, faster, and more efficient, and because it does not require the consumption of large amounts of organic solvents which are both expensive and potentially harmful
The aim of this study is to investigate the effects of extraction time, temperature, pressure and different concentration of ethanol in supercritical CO2 extraction on the yields of amentoflavone, quercetin and ginkgetin (Figure 1) by applying a central composite design method

Summary

Introduction

The flavonoids are one of the largest known groups of natural products, which are widely distributed in various plants [1,2]. Taxus is an important natural resource for the extraction of taxoids, which have been utilized as anticancer agents [7]. Taxus, which is significant to extract the flavonoids for food and pharmaceutical applications [9]. Supercritical CO2 extraction is currently regarded as a natural and green technique for natural product extraction, and an important alternative to conventional separation methods, because it is simpler, faster, and more efficient, and because it does not require the consumption of large amounts of organic solvents which are both expensive and potentially harmful. The technique has been applied to extract bioactive compounds from natural resources [10]

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