Abstract

The synthesis of (+)AuNPs procedure using a stainless-steel mesh was optimized. The optimal synthetic parameters were found to be one piece of stainless-steel mesh (22.5cm2 in surface area) in 100mL of a 1mM precursor, precursor solution pH 4, and reaction temperature of 30°C. Under the optimal conditions, the as-synthesized (+)AuNPs were highly positively charged (+24.2mV). Therefore, the as-synthesized (+)AuNPs act as a peroxidase mimic and provide a simple, fast, highly sensitive and selective colorimetric method for H2O2 detection with a detection limit of 0.06mM in the linear range from 0.06mM to 4.29mM.

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