Abstract
A 1-step extraction process is reported for near quantitative (>96%) extraction of the total lipids from the biomass paste (~72% moisture by weight) of the marine microalga Nannochloropsis salina. The composition of the solvent mixture of chloroform, methanol and water is first optimized for a near quantitative extraction of the lipids. The optimal solvent mixture is found to be chloroform, methanol and water in the volume ratio of 5.7:3:1. This solvent composition is then used in a 1-step extraction process to optimize the extraction time, temperature and the volume of the solvent mixture required for extraction from 1g dry equivalent of the biomass paste. The optimal extraction conditions are found to be 25°C in a 2-h extraction process using 33mL of the solvent mixture. Compared to the commonly used Bligh and Dyer extraction protocol, the proposed 1-step extraction reduced the mixed solvent use by ~48% and the extraction time by ~78%.
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