Optimization of Microwave-assisted Extraction of Flavonoids from &lt;em&gt;Cryptotaenia japonica Hassk&lt;/em&gt; using Response Surface Methodology

Jun Lu,Chi Zhou,Bo Zhou,Yanyan Xu,Ou Rong,Zhonghai Li

doi:10.19026/ajfst.5.3262

Abstract

The objective of the study was to optimize the processing of Microwave-Assisted Extraction (MAE) of flavonoids from <em>Cryptotaenia japonica Hassk</em>. The effects of processing parameters include ethanol proportion, extraction time, extraction temperature and ratio of liquid to solid on the flavonoids yield were determined and the optimal conditions for flavonoids were evaluated by means of Response Surface Methodology (RSM). The optimal conditions were detected and tri-dimensional response surfaces were plotted from the mathematical models. The F-test and p-value showed that all parameters had highly significant effects on the response value. The optimum extraction conditions were ethanol of 70% (v/v), extraction time of 15 min, extraction temperature of 75°C and liquid to solid ratio of 20 mL/g. Under these conditions, 27.01 mg/g total flavonoids were obtained by Microwave- Assisted Extraction (MAE), which are close to the predicted value (27.48 mg/g). MAE method was applied successfully to extract total flavonoids from <em>Cryptotaenia japonica Hassk</em>.

Highlights

Cryptotaenia japonica Hassk (Yajiaoban) is a valuable perennial herb and wildly distributed in forests or shady places of China and Japan
The results indicated that the yield of total flavonoids increased with the increase of Microwave-Assisted Extraction (MAE) time in the beginning of extraction
The result was shown in MAE has advantage of reduction in extraction time, saving energy and increased extraction yield as compared with maceration extraction. These results indicated that the MAE was more suitable for extraction of total flavonoids from Cryptotaenia japonica Hassk than maceration extraction

Summary

Introduction

Cryptotaenia japonica Hassk (Yajiaoban) is a valuable perennial herb and wildly distributed in forests or shady places of China and Japan. Cryptotaenia japonica Hassk is a popular green food in west district of China and it has been used as folk medicine to treat various ailments such as skin itch and traumatic injury (Yang and Xia, 2010). Its main chemical constituents include essential oil such as- (-) -and (+) β-Selinenes, β-and (-) γ-elemenes (Cheng et al, 2010, 2008). Recent studies showed it includes flavonoids (Li et al, 2006; Yao et al, 2010). More and more investigators focus on flavonoids and efficient extraction technique is key issue for its widely application

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Conclusion