Abstract
Dextranase catalyzes the degradation of the substrate dextran, which is a component of plaque biofilm. This enzyme is involved in antiplaque accumulation, which can prevent dental caries. The activity of crude dextranase from Penicillium roquefortii TISTR 3511 was assessed, and the maximum value (7.61 unit/g) was obtained at 37 °C and pH 6. The Plackett–Burman design was used to obtain significant factors for enhancing fungal dextranase production, and three influencing factors were found: Dextran, yeast extract concentration and inoculum age. Subsequently, the significant factors were optimized with the Box–Behnken design, and the most suitable condition for dextranase activity at 30.24 unit/g was achieved with 80 g/L dextran, 30 g/L yeast extract and five day- old inoculum. The use of 0.85% alginate beads for encapsulation exhibited maximum dextranase activity at 25.18 unit/g beads, and this activity was stable in toothpaste for three months of testing. This study explored the potential production of fungal dextranase under optimal conditions and its encapsulation using alginate for the possibility of applying encapsulated dextranase as an additive in toothpaste products for preventing dental caries.
Highlights
Dextranase is an enzyme that catalyzes the endohydrolysis of α-(1-6)-d-glycoside linkages in dextran [1]
Dental plaque or biofilm in the oral cavity contains dextran, which is a product of dental caries pathogens such as Streptococcus mutans and Streptococcus sobrinus [2,3,4]
The crude dextranase activity of strain Thailand Institute of Scientific and Technological Research (TISTR) 3511 was examined for its optimal pH and temperature
Summary
Dextranase is an enzyme that catalyzes the endohydrolysis of α-(1-6)-d-glycoside linkages in dextran [1]. Dental plaque or biofilm in the oral cavity contains dextran, which is a product of dental caries pathogens such as Streptococcus mutans and Streptococcus sobrinus [2,3,4]. It is well recognized that dextranase breaks down the structure of biofilm [5]. Dextranase has received considerable attention in the food, medicine, and dental fields [6]. There are many active ingredients used in different formulations to prevent tooth decay as well. The application of dextranase in oral care is an emerging trend in the world as dextranases can be added in toothpaste or other dental care products to prevent dental caries [1]
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