Abstract

Objective To optimize the flash type extraction of Danzhuye Granules. Methods With the total flavonoids and extract yield as indices, the orthogonal test was adopted to optimize the extraction times, the ethanol concentration, solid-liquid ratio, and the duration of extraction for Danzhuye Granules. Results The factors that infuenced the extract efficiency from high to low were the extraction times, volume fraction of ethanol, the ratio of liquid to solid, and the extraction time. The optimum extracting condition included that the origin was extrated by the ethanol volume fraction 80% with the material to liquid ratio 30:1, total of 3 times, with each time of 3 minutes. Validation experiments of the potimal condition showed that the extraction of total flavonoids was 29.1 mg/g, and the extract yield was 23.6%. Conclusions The flash type extraction method is suitable for extraction, which provides a new method for the development and utilization of Danzhuye Granules. Key words: Orthogonal test; Flavonoids; Flash type extraction; Danzhuye granules; Extract yield

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