Abstract
A moderately halophilic bacterium, Halomonas xianhensis SUR308 (GenBank Accession No. KJ933394) was isolated from multi-pond solar salterns of Odisha, India. Exopolysaccharide (EPS) production by this strain in malt extract yeast extract (MY) medium has been optimized under batch culture system. Among the different media tested, MY medium showed an EPS production of 2.55 g/L, which increased to 2.85 g/L under optimized aeration. An initial pH of 7.5 and incubation temperature of 32 °C were found to be most suitable for EPS production by the isolate under aerobic condition. An EPS production of 3.85 g/L was achieved when the growth medium was supplemented with 2.5% NaCl. Glucose was the most favourable carbon source for EPS production and maximum production (5.70 g/L) was recorded with 3% glucose. However, growth as well as production of EPS was remarkably affected when the growth medium was supplemented with hydrocarbons as sole source of carbon. Among different nitrogen sources, casein hydrolysate at 0.5% level was proved to be the best for EPS production and an initial inoculum dose of 7% (v/v) enhanced the EPS production to 7.78 g/L, while the divalent metal ions were in general toxic to growth and EPS production, EPS synthesis by SUR308 was enhanced with Cr (VI) supplementation.
Highlights
In recent years, increasing attention is being paid to microbial exopolysaccharides (EPS) mainly because of their bioactive role and extensive range of potential applications in modern biotechnology especially in medicine and pharmaceuticals as antiangiogenic or antiviral agents or even in case of targeted drug delivery [1]
Halomonas xianhensis SUR308 (GenBank Accession No KJ933394), a potent EPS producing moderately halophilic bacterium isolated from solar salterns of Surala, Odisha, India was used throughout this study
Growth and EPS production were estimated after 11 days of growth under continuous shaking at 32 °C
Summary
In recent years, increasing attention is being paid to microbial exopolysaccharides (EPS) mainly because of their bioactive role and extensive range of potential applications in modern biotechnology especially in medicine and pharmaceuticals as antiangiogenic or antiviral agents or even in case of targeted drug delivery [1]. EPS from halophiles have stability towards higher temperature, salinity and even pH [2]. These unique properties of EPS produced by halophiles seem to offer diverse applications in various fields of industry [3]. Polysaccharides produced by Halomonas eurihalina [4], H. maura [5] have been described and well characterized. Aqueous solution of EPS synthesized by H. eurihalina has been reported to jellify at acidic pH, while mauran, an anionic, sulfated EPS with high uronic acid composition produced by H. maura are being used for different biotechnological purposes [6,7]. Several others [10,11] have documented EPS production from a number of novel Halomonas spp. isolated from diverse ecological conditions
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