Optimization of Extraction Process and Bioactivities of Polyphenols from Cinnamomum Longepaniculatum by Response Surface Method

Abstract

Based on the results of single factor experiment, Box-Behnken response surface method was used to optimize the extraction process of polyphenols from C. longepaniculatum leaves. The influences of ethanol concentration, liquid to material ratio and extraction temperature on the extraction rate of polyphenols were investigated. The antioxidant and whitening activities of polyphenols from C. longepaniculatum leaves were evaluated by measuring the DPPH· free radical scavenging rate and tyrosinase inhibition rate. The results showed that the optimal extraction conditions were as follows: ethanol concentration was 51%, liquid to material ratio was 30: 1 mL/g, extraction temperature was 71 ℃, the extraction rate of polyphenols from C. longepaniculatum was (1.70 ± 0.01) %. The DPPH free radical of 0.50 mg/mL polyphenols from C. longepaniculatum were 82.70%, and the tyrosinase inhibition rate of 10.00 mg/mL polyphenols from C. longepaniculatum were 91.78%. This shows that C. longepaniculatum leaves have good antioxidant and whitening effects.