Abstract

Saponins from Camellia fascicularis leaves, which ultrasonic-assisted extraction process parameters were optimized with Box–Behnken design and antioxidant activities were assessed, were reported in this study. The highest yield of crude saponins was up to 3.68% when the optimal extraction conditions were ratio of solvent to solid of 52.1 mL/g, methanol concentration of 71.1%, and ultrasonic temperature of 50.3 °C. The crude saponins from C. fascicularis leaves were purified using HP-20 macroporous resin with methanol and three fractions (SCF-1, SCF-2 and SCF-3) were obtained. Subsequently, the in vitro scavenging activity assay showed that the fractions proved significant antioxidant capacities as dose-dependent manner on 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). SCF-1 possessed significantly higher antioxidant activity than that SCF-2 and SCF-3. Overall, ultrasonic-assisted extraction could be effectively used to extract C. fascicularis leaves saponins, which possessed potential antioxidant capabilities and is expected to be developed as food materials.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call