Optimization of Extraction Conditions on the Yield of Rana Chensinensis Egg Oil by Statistical Experimental Designs

Abstract

Rana chensinensis egg oil is extracted from Rana chensinensis. The important variables (extraction temperature, extraction time and the ratio of oviductus ranae egg oil and extraction solvent) were optimized by Box-Behnken central composite design under response surface methodology. The statistical analysis showed that the optimum extraction conditions (extraction temperature 60.62C, extraction time 3.16 h and the ratio of oviductus ranae egg oil and extraction solvent 8.91.) led to a maximum yield (19.3%). The Rana chensinensis egg oil was refined. The developed method has a potential to be used for efficient extraction conditions on the yield of Rana chensinensis egg oil.