Optimization of Experimental Parameters in the Solvent Extraction of Trans-Resveratrol from Pruning Waste of Vitis vinifera, Fetească Neagră Variety

Abstract

The past few decades have seen a marked expansion in market demand for food supplements with therapeutic value. Due to this demand, the recovery of vine waste for obtaining certain phytochemicals or plant synthesized compounds with health-promoting activities can be an important economic component, principally with the agreement of the European Union for resveratrol as a new food ingredient. For the sake of economic capitalization, it is necessary to determine optimum extraction parameters for maximum recovery. In this paper, we have determined the optimum parameters for the solvent extraction of trans-resveratrol from vine prunings. We tested different extraction conditions: 35 different types of solvents, 10 types of solid-to-liquid ratios, 10 extraction times, 10 types of granulosity of the ground material and 7 consecutive extractions on the same material. The optimal parameters determined were: solvent ethanol:diethyl ether 4:1 ratio, 1:35 solid liquid ratio g/mL, 4 days for extraction time, 500 µm–350 µm granulosity of powdered material and one extraction on the material. These findings are confirmed by optimization of extracting parameters according to Box–Behnken design.