Optimization of Enzymatic Saccharification of Alkali Pretreated Parthenium sp. Using Response Surface Methodology.

K Pandiyan,Sarika Rana,Anju Arora,Rameshwar Tiwari,Shashi B Singh,Pawan K S Nain,Surender Singh,Lata Nain

doi:10.1155/2014/764898

K Pandiyan, Sarika Rana + Show 6 more

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https://doi.org/10.1155/2014/764898

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Abstract

Parthenium sp. is a noxious weed which threatens the environment and biodiversity due to its rapid invasion. This lignocellulosic weed was investigated for its potential in biofuel production by subjecting it to mild alkali pretreatment followed by enzymatic saccharification which resulted in significant amount of fermentable sugar yield (76.6%). Optimization of enzymatic hydrolysis variables such as temperature, pH, enzyme, and substrate loading was carried out using central composite design (CCD) in response to surface methodology (RSM) to achieve the maximum saccharification yield. Data obtained from RSM was validated using ANOVA. After the optimization process, a model was proposed with predicted value of 80.08% saccharification yield under optimum conditions which was confirmed by the experimental value of 85.80%. This illustrated a good agreement between predicted and experimental response (saccharification yield). The saccharification yield was enhanced by enzyme loading and reduced by temperature and substrate loading. This study reveals that under optimized condition, sugar yield was significantly increased which was higher than earlier reports and promises the use of Parthenium sp. biomass as a feedstock for bioethanol production.

Highlights

In the last few decades, the demand for alternative fuel sources is accelerated due to the excessive consumption of fossil fuels [1]
The compositional analysis of raw sample revealed that the biomass contains cellulose (308.03 ± 0.6 mg/g); pentosans (164.45 ± 0.2 mg/g), and klason lignin (181.28 ± 1.0 mg/g)
The potential of Parthenium sp. as a source of fermentable sugar for bioethanol production was evaluated by estimating the sugar yield during enzymatic saccharification

Summary

Introduction

In the last few decades, the demand for alternative fuel sources is accelerated due to the excessive consumption of fossil fuels [1]. The utilization of nonfood biomass, that is, lignocellulosic biomass, is creating interest worldwide. The lignocellulosic biomass has the advantage of huge availability, being economical, and reduced emissions of greenhouse gases and does not have the socioeconomic concerns regarding the use of food resources. These factors make them one of the most promising technological approaches available for supplementing the current source of transportation fuel. Effective conversion of recalcitrant lignocellulosic biomass to ethanol includes five subsequential steps: (1) biomass pretreatment, (2) cellulose hydrolysis (saccharification), (3) fermentation of hexoses, (4) separation, and (5) effluent treatment [3]

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