Abstract
A strain of bacillus alkalophilus12-7 which could produce glucanotransferase (CGTase) forming γ-cyclodextrin was screened. Cyclodextrin production technology conditions were studied, and we mainly discussed the influence of enzymatic reaction conditions on γ-cyclodextrin production by glucanotransferase. The results show that enzymatic reaction conditions are pH 8.0, temperature 60°C, enzyme amount 500 U/g starch, glycyrrhizic acid concentration 2%, DE 6 ~ 11, γ- CD yield is good, edible canna starch and tapioca starch are suitable substrate for CGTase in production of γ- CD, pullulanase in increased the γ- CD yield is limited.
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