Optimization of Enzymatic Hydrolysis of Protein from Small Yellow Croaker (&lt;em&gt;Psendosciaena polyactis&lt;/em&gt;) Using Response Surface Methodology

Ying Ji,Yinghui Zhang,Guozeng Zhang

doi:10.19026/ajfst.11.2346

Abstract

Enzymatic hydrolysis of protein from small yellow croaker (Pseudosciaena polyactis) was studied by response surface methodology. Papain hydrolysate was found to have the greatest activity of antioxidant action of hydrolysate on DPPH. A central composite design with four independent variables: time (3, 4 and 5h); enzyme concention (0.2, 0.3 and 0.4%); temperature (50, 55 and 60°C); meal/water ratio (1:3, 1:4 and 1:5) was used to study the response variable (the degree of hydrolysis, DH). From RSM generate model, the optimum conditions for hydrolysis of protein from small yellow croaker were identified to be at temperature 57.75°C in 3.89 h reaction time, with enzyme concentration of 0.22% and meal/water ratio of 1:5. At the optimum conditions, predicted DH in the hydrolysis process was 49.37%.

Highlights

Small yellow croaker (Pseudosciaena polyactis) is an important commercial marine fish species in China and distributed along Yellow Sea, Bohai Sea and East China Sea
Effect of commercial food grade proteases on the degree of hydrolysis: Three commercial food grade proteases were used to hydrolyze the small yellow croaker and each hydrolysis experiment was carried out with a given optimal condition provided by manufacturers (Table 1)
Small yellow croaker protein was effectively hydrolyzed by papain to obtain peptides with a strong antioxidant activity

Summary

Introduction

Small yellow croaker (Pseudosciaena polyactis) is an important commercial marine fish species in China and distributed along Yellow Sea, Bohai Sea and East China Sea. Some small yellow croaker with high production in China is still under utilized despite the fact that they are rich in high quality protein This has highlighted the need to develop value-added products from small yellow croaker with the purpose of maximizing the use of this abundant and low price fish. To produce hydrolysates with desirable properties it is necessary to undertake studies to find the right proteolytic enzyme for a protein substrate. It is, necessary to select factors (enzyme and substrate concentration, time, temperature and pH) with major effects on the proteolysis (Aspmo et al, 2005; Liaset and Espe, 2008; Nilsang et al, 2005; Ren et al, 2008).

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