Abstract
Dispersive liquid–liquid microextraction with high performance liquid chromatography and response surface methodology was developed for preconcentration and determination of dipyridamole in water and urine samples. The effects of different factors on the extraction efficiency were studied simultaneously using experimental design. The variables of interest in the process were extraction and disperser solvent volumes, salt effect, sample volume, centrifuge time, centrifuge speed, and extraction time. An orthogonal array design was performed for screening of variables to determine the significant variables affecting the extraction efficiency. Then, the significant factors were optimized using a fractional factorial design. The optimum experimental conditions found from this statistical evaluation included: sample volume 4 cm3, volume of extraction solvent (chloroform) 60 mm3, volume of disperser solvent (methanol) 0.5 cm3, sodium chloride concentration 2 × 10−4 g cm−3, extraction time 1 min, centrifuge speed 2000 rpm, and centrifuge time 5 min. The method was validated over a concentration range of 4–700 µg dm−3 for urine sample and 0.4–700 µg dm−3 for water sample. The proposed method showed good agreement between the experimental data and predictive value, and it has been successfully employed to determine dipyridamole in water and urine samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
