Abstract
A method was developed to determine 2-mercaptobenzimidazole in water and urine samples using dispersive liquid-liquid microextraction technique coupled with ultraviolet-visible spectrophotometry. It was essential to peruse the effect of all parameters that can likely influence the performance of extraction. The influence of parameters, such as dispersive and extraction solvent volume and sample volume, on dispersive liquid-liquid microextraction was studied. The optimization was carried out by the central composite design method. The central composite design optimization method resulted in 1.10mL dispersive solvent, 138.46μL extraction solvent, and 4.46mL sample volume. Under the optimal terms, the calibration curve was linear over the range of 0.003-0.18 and 0.007-0.18μg/mL in water and urine samples, respectively. The limit of detection and quantification of the proposed approach for 2-mercaptobenzimidazole were 0.013 and 0.044μg/mL in water samples and 0.016 and 0.052μg/mL in urine samples, respectively. The method was successfully applied to determination of 2-mercaptobenzimidazole in urine and water samples.
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