Optimization of D-xylose to xylitol biotransformation by Candida guilliermondii cells permeabilized with Triton X-100

Abstract

The effect of NADP+ and glucose-6-phosphate (G6P) on the biotransformation of D-xylose to xylitol by cells of Candida guilliermondii permeabilized with surfactant Triton X-100 was evaluated. The experimental runs were performed with 12 g L−1 of permeabilized cells and a reaction medium composed of Tris–HCl buffer (0.1 M pH 7), D-xylose (57 g L−1), and MgCl2.6H2O (5 mM). The levels of NADP+ (from 0.0 to 1.7 mM) and G6P (from 0.00 to 0.17 M) were varied according a 22-full factorial composed design. Under optimized conditions (NADP+ 0.5 mM and 0.05 M G6P), the xylitol volumetric productivity (QP) and yield factor (YP/S) predicted were 1.86 ± 0.03 g L−1 h− 1 and 0.64 ± 0.03 g g−1, respectively. These values were 94% and 19% higher than those obtained with unpermeabilized cells under fermentation conditions (0.97 g L−1 h−1 and 0.53 g g−1, respectively). On the basis of the results, it can be concluded that xylitol production by biotransformation with cells of C. guilliermondii permeabilized with Triton X-100 is a promising alternative to the fermentative process.