Optimization of Crystalline Xylose Production from Coffee Cherry Processing Waste

Abstract

Purpose: This study aims to optimize the production of crystalline xylose from coffee cherry processing waste (CCPW) by investigating the influence of substrate concentration (SC), xylanase concentration (XC), and commercial xylose crystal concentration (CXC) on the process. Theoretical Reference: The research utilizes Response Surface Methodology (RSM) to design experiments and analyze the effects of SC, XC, and CXC on crystalline xylose production. Method: Experimental laboratory procedures were conducted with varying levels of SC (10-20%), XC (1-5%), and CXC (2-7%). Xylose production was carried out in fed-batch mode, with substrate feeding every 12 hours. Solvent volume was maintained at 250ml, with 60% added initially and the remaining 40% distributed incrementally during production. Results: The study found significant effects of XC and SC on xylose production, while CXC showed no significant influence. The optimized conditions were determined with SC at 10%, XC at 5%, and CXC at 7%, resulting in a xylose content of 0.804 g/L and a desirability value of 0.632. The model's validity was confirmed with a validation accuracy of 98.99%. Conclusion: The research demonstrates the effectiveness of RSM in optimizing crystalline xylose production from CCPW, highlighting the importance of XC and SC levels in the process. Implications of Research: The optimized conditions identified in this study offer practical insights for enhancing xylose production efficiency from CCPW, potentially contributing to the development of functional food ingredients and process engineering applications. Originality/Value: This study contributes to the field by providing a systematic approach to maximizing xylose production from coffee cherry processing waste, offering valuable knowledge for both academia and industry.