Abstract
The parameters of the immobilized process of Corynebacterium glutamicum VTCC - B - 0632 on alginate were identified by Plackett-Burman matrix, and the experiments were designed by response surface methodology having the central composite designs (RSM-CCD). The maximum yield of cell immobilization on alginate carrier reached at 92.6%. Optimal parameters were the cell density of 89.3 million cells/mL in the 4% sterile alginate with ratio 1:1. This mixture went through the syringe system of the 2M CaCl2 solution at 20 0 C with the shaking speed of 75 rpm until the gels get in shape. Then, these gels were soaked in the CaCl2 liquor and shaken for 41 minutes (150 rpm). At last, the particle size of final products was 4mm and the average cell density was 14.75 million cells/gram. This immobile product is maintained under the suitable condition in the CaCl2 liquor (w/v), pH=7. The cell survival percentage after 72 hours were 98% when it was stored in 4 0 C, 0.5% CaCl2 and pH of 7.
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