Abstract
In this study, polysaccharides of Lyophyllum decastes (LDS) were used as raw material. The chelating rate was set as the index, the effect of chelating time, mass ratio and initial mass concentration on chelating rate was investigated. Response surface method was used to optimize the preparation technology of LDS and Zinc (Ⅱ) chelate (LDS-Zn) based on single factor experiments. LDS-Zn was analyzed by FTIR. Antioxidant activity in vitro of LDS and LDS-Zn was investigated. Research results showed that optimal preparation conditions of LDS-Zn were as follows: Chelating time was 4 h, mass ratio (LDS with zinc) was 6:1, initial mass concentration (zinc) was 4 mg/mL. LDS achieved the best chelating rate as high as 87.08%. Infrared spectroscopy analysis showed that Zinc (Ⅱ) successfully chelated with LDS. Compared with LDS, LDS-Zn had better scavenging effect on DPPH free radicals and hydroxyl radicals with an increase of antioxidant activity by 23.46 and 16.77%, respectively. These results indicated that LDS-Zn can be expected to serve as a nutritional Zn supplement with antioxidant activity.
Highlights
Lyophyllum decastes is a delicious food with medicinal properties (Cheng, 2014)
Some synthetic polysaccharide Zn-chelate included Flammulina velutipe polysaccharide-Zn2+ chelate (Zhao et al, 2016; He and Lu, 2011), Pleurotus eryngii polysaccharide-Zn2+ chelate (Gu et al, 2019), black fungus polysaccharide Zn complex (Li and Wang, 2006), d-glucosamine Zn complex (Raikwar et al, 2019) and chitosan Zn complexes (Ding et al, 2003). These synthetic polysaccharide Zn complexes can be developed into a new Zn supplement (Debon and Tester, 2001; Hedemann et al, 2006; Namkung et al, 2006)
Based on the results of all singlefactor experiments, chelation time, mass ratio and initial mass concentrations were selected as independent variables
Summary
Lyophyllum decastes is a delicious food with medicinal properties (Cheng, 2014). Its fruit body is rich in anticancer polysaccharides and has adjuvant treatment effects on six major diseases, such as diabetes and hypertension. Some synthetic polysaccharide Zn-chelate included Flammulina velutipe polysaccharide-Zn2+ chelate (Zhao et al, 2016; He and Lu, 2011), Pleurotus eryngii polysaccharide-Zn2+ chelate (Gu et al, 2019), black fungus polysaccharide Zn complex (Li and Wang, 2006), d-glucosamine Zn complex (Raikwar et al, 2019) and chitosan Zn complexes (Ding et al, 2003) These synthetic polysaccharide Zn complexes can be developed into a new Zn supplement (Debon and Tester, 2001; Hedemann et al, 2006; Namkung et al, 2006). The study can provide a theoretical basis including preparation and antioxidant activity for the development of a novel natural zinc supplement (LDS-Zn)
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