Abstract
Callus induction from internode explants of Lathyrus sativus L. cv. Nayagarh local was tested in different media (MS, LS, BM, B 5 and W) with different combination of auxins and cytokinins. Overall callus induction frequency and callus growth was very high in B 5 and BM medium. 2, 4-D was found to be effective for callus induction and growth, both in amount and rate, followed by NAA in all media tested. Maximum callusing response was revealed in B5 medium with 2mg/l 2,4-D+0.5mg/l BAP fallowed by B5 + 2mg/l NAA + 0.5mg/l BAP, but the latter most frequently induced greenish white to dark green, loose, nodular and hard calli suitable for plant regeneration and genetic transformation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
