Optimization of B. cereus PW4 alpha amylase production by OVAT technique

Abstract

Amylase enzymes are used in a variety of industries. Palm wine was screened for amylase producing microorganisms by starch iodine method. Isolate PW4 was discovered to be the most effective isolate and was identified as Bacillus cereus PW4 (OK384566) by sequencing the 16S rRNA gene. The one-variable-at-a-time strategy was used to optimize α-amylase production parameters, including pH, temperature, incubation duration, inoculum volume, nitrogen source, and metal ion as factors. The enzyme activity was increased to 141.91 U/ml (5.8-fold) in the optimized medium (pH 7, 35 °C, 72 h, 3 % inoculums, 1 % NH4NO3 and 1 % FeCl3) from 24.0 U/ml in the un-optimized medium. Data of the optimal condition will aid in the scaling up of amylase production and other industrial activities.