Abstract

Halophilic actinomycete Nocardiopsis sp. DW-4 was isolated from Dwarka, the coastal region of Gujarat. The isolate was studied for the extracellular amylase production. The DW-4 strain displayed diversity with respect to growth patterns and production of extracellular amylase. ‘One factor variable at time’ method was used to obtain maximum amylase production. The influence of starch, salt and pH was studied on the amylase production and growth of DW-4. The isolate DW-4 could grow and produce amylase at 5-15% salt and starch 1-3% and produced optimum amylase at 5% NaCl and pH 8. Optimization of the amylase production in conjunction with the growth of the organisms is significant.

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