Abstract
Serratia marcescensPT6 is a Gram-negative bacteria isolated from shrimp pond sediment that capable of producing chitinase. This study aimed to observe the effect of agitation rate on growth and chitinase activity ofS. marcescensPT-6 in a bioreactor. The production of chitinase was done in 1.5 l bioreactor using colloidal chitin broth at the condition of pH 7, the temperature of 30°C, aeration of 0.04 vvm, and variation of agitation rate (200, 350, 500 rpm). Bacterial growth was measured by colonies counting in agar medium, while chitinase activity was measured by means of colorimetric every day for four days incubation. The results of ANOVA analysis show that the agitation rate had no effect on bacterial growth, but a significant effect (P<0.05) was observed on chitinase activity. The highest growth and chitinase activity were obtained at 200 rpm, with the highest chitinase activity of 0.006 ± 0.001 U/ml was at day-2. This study implies that the optimized agitation rate in the bioreactor increased the chitinase activity produced byS. marcescens PT-6.
Highlights
Bioconversion of chitin from shrimp shell waste into N-acetylglucosamine (NAG) is beneficial for the production of a value-added compound derived from seafood waste
This study aimed to determine the effect of agitation rate in the 1.5 l bioreactor on chitinase activity of S. marcescens PT-6
Nutrient broth (NB) and nutrient agar (NA) (Oxoid) medium were used for the preparation of inoculum and cell count, respectively
Summary
Bioconversion of chitin from shrimp shell waste into N-acetylglucosamine (NAG) is beneficial for the production of a value-added compound derived from seafood waste. Chitinase is an enzyme used in the biological process for NAG production. S. marcescens PT-6 was isolated previously from shrimp pond sediments and showed the chitinase activity of 0.0002 U/ml [3, 4]. Optimization of S. marcescens PT-6 growth environment was carried out to increase the production of chitinase [5, 6]. An increase in chitinase activity of 103.5 times was observed by the addition of starch and yeast extract in the production medium [6]. A lab-scale bioreactor is often used to increase the production volume of microbial metabolite products
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