培養基對木黴菌（Trichoderma harzianum）發酵生產幾丁質酵素的影響

Abstract

Chitinase can degrade colloidal chitin to produce biofunctional oligosaccharides. This study was to observe the influences of carbon and nitrogen sources on the production of chitinase by Trichoderma harzianum. The chitinase activities were analyzed by a fluorescence meter after a four-day cultivation. The results demonstrated that colloidal chitin could induce the production of chitinase, but glucose and chitosan couldn't during the Trichoderma harzianum fermentation. Different concentrations (0.5%, 1.0%, 1.5% and 2%) of colloidal chitin applied in the culture media were used to compare their ability in the chitinase induction; the results reveal that the 2% chitin induced the highest chitinase activity, but there were no appreciable difference for the other factors. Comparison among the three different nitrogen sources: yeast extract, malt extract and potassium nitrate/ ammonium chloride, demonstrated that the yeast extract and inorganic nitrogen compounds induced higher chitinase activities than that of the malt extract. The chitinase produced in the media with 2% nitrogen sources showed higher chitinase activity; however, the activities of chitinase were not proportional neither to the carbon concentrations nor to the nitrogen concentrations in the culture media.