Abstract
Background Malaria is responsible for more than 1 million deaths per year; most of the victims are young African children [1]. Recent studies have found that Plasmodium falciparum DNA is detectable in human saliva as in human blood [2]. However the nature of the source of the saliva fraction that serve as source of this DNA still unclear. Thus this study sought to establish the constituents of human saliva that harbours parasite the DNA in malaria infected subjects.
Highlights
Malaria is responsible for more than 1 million deaths per year; most of the victims are young African children [1]
DNA was extracted using the Qiagen DNEasy® kit on different saliva fractions from 46 malaria positive individuals and 42 malaria negative individuals were used in this study
This study further confirms that saliva is a reliable source for detecting malaria based methods and could potential be used as an alternative non-blood based and non invasive malaria detection assay
Summary
Malaria is responsible for more than 1 million deaths per year; most of the victims are young African children [1]. Method To determine whether the source of malaria amplifiable DNA in saliva was intracellular or free DNA in saliva, different saliva extractions were used to extract DNA; i. E. the pellet only , supernatant only and pellet and supernatant mixture.
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