Abstract
Extraction process was optimized by single factor and orthogonal experiment (L_9 (3^4)). Moreover, the content determination was studied in methodology. The optimum ultrasonic extraction conditions were: ethanol concentration of 75%, ultrasonic power of 420 w, the solid-liquid ratio of 1:15, extraction duration of 45 min, extraction temperature of 90°C and extraction for 2 times. Saponins content in Guazhou samples was significantly higher than those in Xinjiang and Inner Mongolia. Meanwhile, Guazhou samples harvested in April and May were higher than that in Guazhou ＂Cynomorium songaricum＂ numbered ＂the three-nine＂. In addition, saponin content in various samples grown in separate years is different. This ultrasonic extraction process can significantly improve the saponins extraction efficiency. Determination by this method is fast, easy-to-operate. The result of this method is reliable. The conclusion of study could provide scientific basis for rational development and quality control of ＂Cynomorium songaricum Rupr＂.
Highlights
In recent years, Cynomorium songaricum Rupr. has been playing core role in health-care and has become a new material for developing and producing health foods and medicines (Zhao et al, 2010)
There have been a few study of Cynomorium songaricum Rupr. saponins; in addition, literature concerning extraction as well as determination content in cs saponins has not been reported (Zhao et al, 2010; Wang et al, 2014; Yoo et al, 2014; Capote and de Castro, 2007)
In this study, ultrasonic extraction was applied for extraction of cs saponins and the optimal conditions of extraction process were investigated by orthogonal experiment
Summary
Cynomorium songaricum Rupr. (cs) has been playing core role in health-care and has become a new material for developing and producing health foods and medicines (Zhao et al, 2010). (cs) has been playing core role in health-care and has become a new material for developing and producing health foods and medicines (Zhao et al, 2010). There have been a few study of Cynomorium songaricum Rupr. Saponins (cs saponins); in addition, literature concerning extraction as well as determination content in cs saponins has not been reported (Zhao et al, 2010; Wang et al, 2014; Yoo et al, 2014; Capote and de Castro, 2007). The cs saponins content in different samples from various origins were determined in this study to provide basis for establishment extraction methods, content determination together with quality standard of cs saponins (Nickrent et al, 2005)
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