Abstract
The objective of this investigation was the optimization of the immobilization of naringinase from Penicillium decumbens by entrapment in poly(vinyl alcohol) and polyethylene glycol cryogels. First, the effect of the characteristics of the matrix components on immobilization efficiency and mechanical stability of the biocatalyst obtained, was determined. Experimental design and response surface methodology was used to analyse the effect of pH (3.0-8.0), the concentrations of poly(vinyl alcohol) (5.3-8.7%) and polyethylene glycol (7.0-12.0%) on the immobilization efficiency, and to optimize these parameters. Additionally, reuse capacity in synthetic juices of immobilized enzyme was studied. The immobilized biocatalysts obtained, conserved 45-47% of active enzyme, and, after three cycles of reuse in synthetic juice, retained 54.3% of their initial activity. Results showed that immobilization efficiency and operational stability of entrapped naringinase were affected by immobilization conditions.
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