Abstract
Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.
Highlights
Using renewable energy sources to develop energy production and environmentally friendly materials is essential for decreasing fossil fuel use and overcoming growingMicrobial lipids obtained from oleaginous microorganisms have received considerable attention in recent years because their chemical components are similar to plant-based lipids used for biodiesel production (Christophe et al 2012; Ye et al AMB Express (2021) 11:149Yen et al 2015)
In order to investigate the effect of ammonium nitrate on yeast growth and lipids accumulation, and to compare with yeast cultured with ammonium sulphate as the nitrogen source, we explored using 4% sucrose as the carbon source, ammonium nitrate as the nitrogen source, and assessed the effect of different C/N ratios on the lipids production characteristics of yeast
The growth of lipids-producing microorganisms and some metabolic activities are affected by the different carbon sources and the amount of carbon source added in the medium (Gong et al 2019)
Summary
Using renewable energy sources to develop energy production and environmentally friendly materials is essential for decreasing fossil fuel use and overcoming growingMicrobial lipids obtained from oleaginous microorganisms (e.g., yeast, bacteria and algae) have received considerable attention in recent years because their chemical components are similar to plant-based lipids used for biodiesel production (Christophe et al 2012; Ye et al AMB Express (2021) 11:149Yen et al 2015). Lipomyces sp.) are considered desirable microbial lipids producers due to their ability to accumulate large quantities of lipids, achieve faster growth, adapt to a wide range of carbon sources, and withstand environmental changes (Liu et al 2020; Qi et al 2020). There are relatively few studies on the production of microbial lipids by R. toruloides 2.1389 using different nutrients (carbon sources, nitrogen sources, and C/N ratios), and no systematic evaluation of the changes in biodiesel characteristics caused by nutritional differences (Braunwald et al 2013; Lopes et al 2020; Saini et al 2021)
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