Abstract
Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.
Highlights
Lawsonia inermis L., commonly known as henna, is an important industrial and medicinal plant
The mean number of shoots obtained in our research was relatively low (3.8), but similar results (3.2 - 4.5) were obtained by Ro u t et al (2001) on the MS medium containing 0.25 mg.L-1 BAP and 0.25 mg.L-1 K i n et i n
Rooting percentage obtained in our research on a half-strength MS medium with 1.0 mg.L-1 IBA was 70%, while Ro u t et al (2001) recorded the highest number of 75% rooted shoots on an MS medium with 0.25 mg.L-1 IBA, with a decreasing rooting rate on media with higher concentrations of IBA
Summary
Lawsonia inermis L., commonly known as henna, is an important industrial and medicinal plant. It is cultivated mostly for a dye production which is derived from henna leaves and used in a cosmetic industry (Zafa r et al, 2006). R a m , S h ekh awat (2011) reported an improved protocol for the micropropagation of henna using nodal explants obtained from mature plants. They successfully performed ex vitro rooting of obtained
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