Abstract
Abstract Optimal concentration of substrate, α-oxobutyrate, for determination of α-hydroxybutyrate dehydrogenase (HBD) activity in normal and pathological sera at 30 °C (with a centrifugal analyzer) is 12 mmol/ liter. With this substrate concentration, HBD activity averaged 146% of that found with the suboptimal concentration, 3.3 mmol/liter, usually used. Furthermore, day-to-day precision was significantly better. HBD/LD ranges (LD, lactate dehydrogenase), determined for sera from patients with myocardial infarction and liver disease, did not overlap and were similar to those established by Elliot et al. [Clin. Sci. 23, 305 (1962)], who used a suboptimal α-oxobutyrate concentration of 3.3 mmol/liter at 25 °C. At 30 °C the mean HBD/LD ratios for sera from patients with myocardial infarction were most different from those for patients with liver disease when the optimal concentration of α-oxobutyrate was used.
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