Abstract
The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain ofPseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from carwash wastewater. The purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.Influence of different Physicochemical factors such as nitrogen and carbon sources, pH,temperature, inoculation percent and different concentrations of SDS on the biodegradation ofSDS were investigated by measuring the degradation rate of SDS using methylene blue activesubstance (MBAS) method. The optimum conditions determined for the this selected bacteriumstrain for degradation of SDS were 1.5mM SDS, inoculation percent 7%, pH 7.5, temperature37°C, ammonium nitrate (nitrogen source) when basal salt medium was supplemented withglucose as a co substrate. This bacterium is able to degrade about 98% of the SDS after 24h ofincubation under optimized conditions of biodegradation. The results presented in this researchindicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation.
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