Optimal blue light irradiation conditions for the treatment of acne vulgaris in a mouse model

Abstract

Although blue light is one of the therapeutic approaches used to treat acne vulgaris (AV), there is no consensus on its effectiveness. As a result, it is not recommended in the major acne vulgaris treatment guidelines. The goal of this study was to look into the mechanism, safety, and efficacy of blue light therapy. We achieved this by examining the pathological response, inflammation, and depth of light penetration in a mouse model of cystic AV. The aims of the study were addressed by exposing the mice to light with a wavelength of 415nm under four different irradiation conditions. The exposure was done for five consecutive days followed by a no irradiation period of 72h. Blue light treatment was most effective when irradiation was performed at 100mW/cm2 for 20min for five consecutive days. Inflammatory responses emerged 72h after the final irradiation dose was administered. These responses were not associated with apoptosis as cleaved caspase-3 staining revealed no significant increases in apoptosis in the skin under any of the tested conditions. Blue light reached the superficial layer of the acne cyst at 5% of the total irradiation power and was attenuated by half for every 50μm of progress through the cyst. In conclusion, blue light could control severe dermatologic inflammatory responses; therefore, it can be used to irradiate AV with high inflammation levels on a daily basis until improvement is observed. In addition, porphyrin, a metabolite of Cutibacterium acnes, and reactive oxygen species generated by the surrounding skin tissue may have essential roles in AV treatment.