Abstract
For the chemotaxonomy of actinomycetes, we describe the optical resolution method for 2, 6-diaminopimelic acid (DAP) stereoisomers by high performance liquid chromatography (HPLC) using 2, 3, 4, 6-tetra-O-acetyl- β-D-glucopyranosyl isothiocyanate (GITC). The analysis was applicable both to whole-cell preparations and to cell wall ones. This method made it possible to analyze quantitatively LL-, DD-, and meso-DAP, compared with the previously reported methods of paper chromatography, thin-layer chromatography and HPLC.
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