Abstract
The observation of fluorescence in a tissue offers a method of real-time monitoring of chemical reactions when one of the reagents or products in fluorescent. This paper discusses how tissue optics affects one's observation of fluorescence in a turbid medium such as tissue. An experimental example is also shown: the photobleaching of a photosensitizer molecule (protoporphyrin IX) within rat tumors. A simple algorithm (Gardner et al., 1993) is given which enables the corrections for tissue optics.
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