Abstract
Dual-comb microscopy (DCM), based on a combination of dual-comb spectroscopy (DCS) with two-dimensional spectral encoding (2D-SE), is a promising method for scan-less confocal laser microscopy giving an amplitude and phase image contrast with the confocality. However, signal loss in a 2D-SE optical system hampers increase in image acquisition rate due to decreased signal-to-noise ratio. In this article, we demonstrated optical image amplification in DCM with an erbium-doped fiber amplifier (EDFA). Combined use of the image-encoded DCS interferogram and the EDFA benefits from not only the batch amplification of amplitude and phase images but also significant rejection of amplified spontaneous emission (ASE) background. Effectiveness of the optical-image-amplified DCM is highlighted in the single-shot quantitative nanometer-order surface topography and the real-time movie of polystyrene beads dynamics under water convection. The proposed method will be a powerful tool for real-time observation of surface topography and fast dynamic phenomena.
Highlights
Dual-comb microscopy (DCM), based on a combination of dual-comb spectroscopy (DCS) with twodimensional spectral encoding (2D-SE), is a promising method for scan-less confocal laser microscopy giving an amplitude and phase image contrast with the confocality
After the image-encoded optical frequency comb (OFC) beam was combined with the reference OFC beam with a time separation of 6.2 ns by beam splitter (BS) again, it was fed into the experimental setup of the DCS
We demonstrated effectiveness of optical image amplification in DCM for enhanced quality of confocal amplitude and phase images in the rapid acquisition rate or weak signal acquisition
Summary
Dual-comb microscopy (DCM), based on a combination of dual-comb spectroscopy (DCS) with twodimensional spectral encoding (2D-SE), is a promising method for scan-less confocal laser microscopy giving an amplitude and phase image contrast with the confocality. Due to the scan-less imaging capability in DCI and the simultaneous acquisition capability of amplitude and phase spectra in DCS, combination of DCI with confocal laser microscopy enables the scan-less confocal one-dimensional (1D)[21,23,24,25,27] or two-dimensional (2D)[22,26] imaging of amplitude and phase Such dual-comb microscopy (DCM) has been effectively applied for the surface topography of a nanometer-scale step-structured sample and the non-staining imaging of standing culture fixed cells[22]. The SNR and contrast in confocal amplitude and phase images were significantly enhanced without influence of incoherent background light of amplified spontaneous emission (ASE) by coherent amplification of image-encoded OFC interferogram in erbium-doped fiber amplifier (EDFA)
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