Abstract
The content of biogenic amines has been studied due to the toxicity of these compounds in humans when are consumed exogenously, and is used as an indicator of quality in the food industry. The manly method for its determination is high performance liquid chromatography. However, it requires a long time for analysis. An alternative method is the use of biosensors, porous silicon with gold nanoparticles were obtained by electrochemical attack assisted with metal salt. These substrates were used for the construction of a biosensor capable of detecting BA using diamine oxidase enzyme as an element of biological recognition and is binding using the self-assembled monolayers method. The correlation between scanning electron microscopy, X-ray dispersive energy spectroscopy, and Fourier Transform infrared spectroscopy analysis, demonstrated the correct construction of the biosensor and the detection of biogenic amines by interaction with the enzyme. Finally, with the built biosensor it was possible to detect three important biogenic amines found in food.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.