Abstract
Abstract Determination of biogenic amines in invertebrates, by means of high-performance liquid chromatography (HPLC), is of considerable physiological significance. In insects, octopamine is released into the hemolymph in response to stress, and exhibits some myogenic properties in both insects and nematodes. The first part of the present paper deals with the synthesis of N-acetyloctopamine from octopamine and with its characterization. The second part deals, for the first time, with the detection of these biogenic amines in insects and nematodes using the chromatographic system with electrochemical detection. N-acetyloctopamine was detected in a chromatographic system enabling determination in biological samples. The interest of the used method is in the simultaneous detection of various biogenic amines. Improvements can certainly be made for detection of more specific to invertebrates compounds such as tyramine, octopamine and N-acetylated derivatives.
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