OPPORTUNISTIC BACTERIA: PSEUDOMONAS PUTIDA

Abstract

Background. This work aims to identify bacterial strains of Pseudomonas putida; opportunistic bacteria in groundwater in Algeria and to try to explain why their numbers are increasing this year compared to the previous year. Materials and Methods. 46 groundwater samples were taken during the period from july 2016/ march 2017 at the level of 18 cities and 210 groundwater samples were taken during the period of january 2017 / october 2018 (32 cities in Algeria). Water was analyzed by the membrane filtration technique. After incubation, the characteristic colonies are identified by biochemical tests and 20 NE biochemical gallery. Results and Discussion. We saw an increase number of Pseudomonas putida in the last and current year, the value is almost 1100 ufc / 250ml. An increase that can be explained by an optimal temperature between 25° C and 30° C and an optimal pH which is between 4 to 8. The use of P. putida strains in industrial processes, commercial products or consumer products may explain the high number of these bacterial strains in groundwater in Algeria. Conclusion. Pseudomonas putida strains appear to be closely related to Pseudomonas fluorescens and that molecular biology techniques must be used to properly identify these two types of bacteria and to construct a phylogenetic tree of strains. Pseudomonas putida strains have no negative effect on the environment or on biological diversity; or endanger the environment that is essential to life and human health. Exposed the strains of P.putida bacteria for a long time to biocides and antibiotics may constitute a battery of defense mechanisms regardless of their targets of action.