Abstract
1. 1. Using tritiated ligands [ 3H]DADLE, [ 3H]DAGO, [ 3H]EKC and [ 3H]Bremazocine, we have demonstrated the presence of δ, μ and κ sites in brain membranes from jerboa ( Jaculus orientalis), a desert rodent and a true hibernator. 2. 2. A comparative study was realized in the case of the induced hibernating state, indicating a reduction of binding capacities during the hibernation state. 3. 3. Using radioimmunoassay, the endogenous pentapeptide methionine-enkephalin (Met-enk) was evaluated in different areas of jerboa brain in comparison with the effect of induced hibernation on the level of Met-enk. 4. 4. A thermodynamic analysis of the effect of temperature on the binding of opioids, indicates that the hibernating and the active state are energetically different. ΔG°, ΔH° and ΔS° were calculated. A break of the Van't Hoff plot was observed in the active state at 15°C, indicating a possible transition state of membranous phospholipids and/or proteins. 5. 5. The role of phospholipids was studied, using the effect of phospholipase A 2 and membrane reconstitution. Phospholipids play a key role in the opioid binding.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call