Open‐tubular supercritical fluid chromatography with simultaneous flame ionization and chemiluminescent nitrogen detection

Abstract

AbstractThe use of a simultaneous detection system for open‐tubular supercritical fluid chromatogrphic separation of aniline isomers, alkyl(C8 to C18)dimethylamines, a dialkylmethylamine mixture, and an extract of a Japanese horseradish power “Wasabi” is demonstrated. A chemiluminescent nitrogen detector (CLND) and a flame ionization detector (FID) were configured at the end of the open tubular column via a zero‐ to dead‐volume tee. The dual detection was achieved with a post‐column split at a 1:2.5 ratio for the CLND and FID, respectively. The optimized CLND showed virtually no response to non‐nitrogenous components such as benzene, toluene, and methanol which were used as injection solvents in these applications.