Gas chromatography-chemiluminescent nitrogen detection: GC-CLND

Abstract

Publisher Summary The chemiluminescent nitrogen detection (CLND) mechanism for chromatography is the same as the total nitrogen detection. For GC-CLND, sample components are eluted from the column and then oxidized at high temperatures (1000o–1100oC). The ٠NO and O 3 chemiluminescence detected by the PMT is proportional to the amount of each nitrogen containing compound eluting from the chromatographic column. In order to obtain the maximum benefit of the CLND, the chromatographer must quantitatively get the nitrogen containing analytes to the detector. Therefore, it is very important that (1) sample injection system, such as split/splitless, cool on-column, or valve (sample loop) injection and transfer lines etc., (2) capillary column (also consider: stationary phase, film thickness, column I,D., and length etc.), and (3) interface to the detector (CLND pyro-furnace and transfer line) are clean and fully operational for the type of sample to be analyzed. Although this is very basic, it is the criteria for every successful chromatographic application using any detector and certainly applies to the CLND.