Oocyte development and vitellogenin production in Northwest Atlantic Greenland halibut Reinhardtius hippoglossoides

Abstract

Abstract Histological data presented here supports the notion of an uncommon oocyte development pattern for Greenland halibut, with two simultaneous groups of developing oocytes in the ovary, the larger group developing for the upcoming spawning season and the smaller group developing for next year ( i.e. the duration of vitellogenesis is > 1 yr.). We analyzed blood samples collected from wild fish as well as fish that were part of the first documented study of Greenland halibut reproductive development in captivity in order to explore the annual cycle of vitellogenin production for this species. Despite the unusual oocyte development strategy there were no obvious differences in seasonal vitellogenin profiles versus fish with more traditional oocyte development strategies. Blood plasma vitellogenin levels generally peaked prior to or during spawning for those fish that successfully spawned, while patterns were highly variable for fish that did not spawn. Maximum plasma vitellogenin levels observed were 25 mg/ml and within the range of values observed for other fish species. Continued refinements in husbandry and experimental protocols for the study of Greenland halibut in captivity will provide a valuable tool for examining aspects of the species’ biology that are difficult to ascertain based on sampling of only wild fish.