Ontogeny-dependent effects of elevated CO2 and watering frequency on interaction between Aristolochia contorta and its herbivores

Abstract

Effects of environmental change on plants can differ due to sequential changes in their life-history strategies (i.e., ontogenetic variations). The fitness of herbivorous insects by physiological changes of the host plant could be affected depending on their diet breadth. However, little is known regarding the combinational effects of plant ontogeny and climate change on plant-herbivore interactions. This study examined the plant ontogeny–dependent effects of climate change on the interaction between a host plant (Aristolochia contorta), its specialist herbivore (Sericinus montela), and a generalist herbivore (Spodoptera exigua). Plants were grown under a factorial design of two distinct CO2 concentrations (ambient, 400 ppm; elevated, 560 ppm) and two watering frequencies (control, once a week; increased, twice a week). Plant ontogeny ameliorated the effects of climate change by altering its defensive traits, where nutrient-related factors were cumulatively affected by climate change. Herbivore performance was assessed at three different plant ontogenetic stages (1st-year juvenile, 1st-year senescence, and 2nd-year juvenile). Elevated CO2 levels reduced the growth and survival of the specialist herbivore, whereas increased watering frequency partially alleviated this reduced performance. Generalist herbivore performance slightly increased under elevated CO2 levels with progressing ontogenetic stages. The effects of climate change, both elevated CO2 and increased watering frequency were weaker in 2nd-year juveniles than in 1st-year juveniles. Elevated CO2 levels detrimentally affected the nutritional quality of A. contorta leaves. The effects of climate change on both specialist and generalist herbivore performance differed as plant ontogenetic stage proceeded. Increased growth rates and survival of the generalist herbivore at the latter ontogenetic stage might negatively affect the population dynamics of a specialist herbivore. This study suggests that biases are possible when the plant-herbivore interaction under a changing environment is predicted from a singular plant ontogenetic stage.