Abstract
This study aimed to express an inulinase gene from the yeast Kluyveromyces marxianus (KmINU) in Aurantiochytrium sp. and realized one-step utilization of inulin resource for DHA production without any chemical pretreatment. An expression cassette with a length of 6052bp for expressing the inulinase gene was constructed by a fast two-step PCR method and then was transferred into the Aurantiochytrium sp. cells. The Aurantiochytrium sp. recombinant T39 was selected with an inulinase activity up to 50.1 U/mL in 72h. In a 5-l fed-batch fermentation, as high as 148.9g/L of inulin was directly used within 120h, and only 1.2g/L of total sugar was left in the medium at the end of fermentation. The biomass of 51.4g/L with a lipid content of 69.2% DCW and a DHA yield of 14.9g/L was obtained.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call