One-step synthesis of nitrogen-doped multi-emission carbon dots and their fluorescent sensing in HClO and cellular imaging.

Abstract

Tunable multicolor carbon dots (CDs) with a quantum yield reach up to 35% were generated directly from rhodamine and urea via one-step hydrothermal approach and purified through silica gel column chromatography. Transmission electron microscopy images reveal that the as-prepared CDs possess a small size distribution below 10nm with bright blue, green, and yellow color emission, designated as b-CDs, g-CDs, and y-CDs, respectively. The in-depth investigations reveal that the multicolor emission CDs with different fraction displays fluorescence emission wavelength ranges from 398nm (b-CDs), 525nm (g-CDs), to 553nm (y-CDs) which could be well modulated by controlling the amount of heteroatom nitrogen especially amino nitrogen onto their surface structures. Further experiments verify the important role of nitrogen content by using rhodamine solely or substituting urea with sulfur containing compounds as precursors to produce corresponding CDs since the performance is lower than that of urea incorporation. Theoretical calculation results also reveal that the increasing amount of amino nitrogen into their surface structures of b-CDs, g-CDs to y-CDs is responsible for reduced band gaps energy, which result in the redshifted wavelength. Benefiting from the excellent photoluminescence properties, wide pH variation range, high photo stability, and low toxicity, these CDs were employed for HClO sensing at 553nm within the range 5 to 140μM with a limit of detection (LOD) of 0.27 ± 0.025μM (n = 3) and multicolor cellular imaging in HeLa cells. Tunable multicolor carbon dots (CDs) were generated directly from rhodamine and urea via one-step hydrothermal approach and purified through silica gel column chromatography. The as-prepared CDs exhibit bright blue, green, and yellow color emission which could be well modulated by controlling the increasing incorporation of heteroatom nitrogen especially amino nitrogen into their surface structures. These CDs were employed for HClO sensing and demonstrated to multicolor cellular imaging in HeLa cells.