Abstract
In this work, a novel type of mesoporous magnetic nanocomposite (Fe3O4@PDA@TiO2@Cu3(BTC)2) was prepared by one-step method with ionic liquid (ILs) as solvent. ILs was used as templates for fabrication the porous material as well as green solvent. The material showed good sensitivity (1 fmol), excellent selectivity (molar ratio of phosphopeptides/non-phosphopeptides up to1:1000) and good repeatability (5 cycles) towards phosphopeptides. Furthermore, it has been applied to the enrichment of phosphopeptides in human saliva and human serum, 29 and 4 endogenous phosphopeptides were detected, respectively.
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