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Abstract
Recently, the incidence of Candida infections has substantially increased. Conventional identification methods for Candida species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously eight medically important Candida species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important Candida species. These primers were able to distinguish each Candida species and did not display cross-reactivity with representative Candida species other than the eight Candida species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.
Highlights
The genus Candida belongs to the kingdom Fungi, class Deuteromycetes, and comprises 150 - 200 species
Because several non-C. albicans Candida species are frequently resistant to common antifungal agents, accurate identification methods are essential for the establishment of appropriate antifungal therapy [11]
Sixteen specific primers covering the upstream regions of intergenic spacer (IGS) and internal transcribed spacer (ITS) gene sequences of eight medically important Candida species were designed in the present study (Figure 1 and Figure 2)
Summary
The genus Candida belongs to the kingdom Fungi, class Deuteromycetes, and comprises 150 - 200 species. Candida albicans remains the most frequent cause of candidemia and hematogenously disseminated candidiasis, an increasing number of hospital-acquired infections caused by other Candida species, so-called non-albicans Candida species, is being observed [1] [2] [3] [4] [5]. There has been a significant upward trend in the emergence of non-albicans Candida species, especially Candida glabrata, Candida parapsilosis, and Candida tropicalis [8] [9] [10]. Because several non-C. albicans Candida species are frequently resistant to common antifungal agents, accurate identification methods are essential for the establishment of appropriate antifungal therapy [11]
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