Abstract
Objective To introduce one method to improve the effect of bone RNA extraction when the classical Trizol method can not meet the experimental requirements.Methods The concentration,purity and integrity of the extracted RNA were tested.The RNA was reversely transcripted into cDNA,as a template which was then amplified to DNA.Results The re-extracted RNA could be fully dissolved by diethyl chlorophosphate (DEPC) water.This method could effectively reduce the interference of mucin carbohydrate.After the prevention of the sodium mixture solution,the concentration of the RNA was slightly lower than that before the prevention.The rate of A260/A280 and A260/A230 of RNA was both significantly higher afher the prevention than that before the prevention [(1.96 ± 0.11) vs.(1.52 ± 0.05),(1.90 ±0.21) vs.(0.27 ±0.08) respectively].Agarose gel electrophoresis showed the RNA after prevention was complete and the polymerase chain reaction (PCR) procession could successfully amplify the targeted gene.Conclusion The improved extraction method could insure the purity,concentration and integrity of RNA,and meet the requirements for molecular research. Key words: Bone tissue; RNA extraction; Rats
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